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Anti-Synaptotagmin-7 Antibody FL490 Conjugate

Our Anti-Synaptotagmin-7 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N275/14. It detects mouse and rat Synaptotagmin-7, and is purified by Protein A chromatography. It is great for use in IHC, ICC.

Product Specifications

Background

Synaptotagmins are a family of calcium sensing, membrane trafficking proteins. 17 different synaptotagmins have been identified in mammals. Synaptotagmin 7 is highly, ubiquitously expressed in the brain and was originally identified as a Ca2+ sensor for lysosomal exocytosis, but has since been demonstrated to play roles in secretion from many different cell types (MacDougall DD, et al., 2018)

Short Description

Our Anti-Synaptotagmin-7 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N275/14. It detects mouse and rat Synaptotagmin-7, and is purified by protein a chromatography. It is great for use in IHC, ICC.

Synonyms

Synaptotagmin-7 (Synaptotagmin VII) (SytVII)

Gene Name

Syt7

UniProt

Q9R0N7

Host

Mouse

Reactivity

Mouse and Rat

Immunogen

Fusion protein amino acids 150-239 (cytoplasmic C2A domain) of mouse Synaptotagmin-7 (accession number Q9R0N7) produced recombinantly in E. Coli

Target

Synaptotagmin-7

Clonality

Monoclonal

Isotype

IgG2b

Clone

N275/14

Type

Primary Antibody

Applications

IHC, ICC

Field of Research

Vesicle Trafficking/Fusion

Concentration

0.5 mg/mL

Format

Purified by Protein A chromatography

Buffer

PBS with 0.09% azide

Molecular Weight

45 kDa (plus others)

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and conjugation of purified mAb. Purified mAbs are >90% specific antibody.

Specificity

Does not cross-react with Synaptotagmin-6 (or other Synaptotagmins)

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Mouse

WB Brain Quality Control

1

Conjugate

FL490 Ex: 491 nm, Em: 515 nm

RRID

AB_2939952

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