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Anti-Synaptotagmin-10 Antibody FL490 Conjugate

Our Anti-Synaptotagmin-10 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N269/73. It detects mouse and rat Synaptotagmin-10, and is purified by Protein A chromatography. It is great for use in IHC, ICC.

Product Specifications

Background

Synaptotagmins are a family of calcium sensing, membrane trafficking proteins. 17 different synaptotagmins have been identified in mammals. Synaptotagmin 10 has been shown to function as the Ca2+-sensor that triggers IGF-1 exocytosis in olfactory bulb neurons (Cao P. et al., 2011) .

Short Description

Our Anti-Synaptotagmin-10 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N269/73. It detects mouse and rat Synaptotagmin-10, and is purified by protein a chromatography. It is great for use in IHC, ICC.

Synonyms

Synaptotagmin-10 (Synaptotagmin X) (SytX)

Gene Name

Syt10

UniProt

Q9R0N4

Host

Mouse

Reactivity

Mouse and Rat

Immunogen

Fusion protein amino acids 248-335 (cytoplasmic C2A domain) of mouse Synaptotagmin-10 (accession number Q9R0N4) produced recombinantly in E. Coli

Target

Synaptotagmin-10

Clonality

Monoclonal

Isotype

IgG1

Clone

N269/73

Type

Primary Antibody

Applications

IHC, ICC

Field of Research

Vesicle Trafficking/Fusion

Concentration

0.5 mg/mL

Format

Purified by Protein A chromatography

Buffer

PBS with 0.09% azide

Molecular Weight

60 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and conjugation of purified mAb. Purified mAbs are >90% specific antibody.

Specificity

Does not cross-react with Synaptotagmin-6 or Synaptotagmin-3

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Mouse

ICC Quality Control

1

Conjugate

FL490 Ex: 491 nm, Em: 515 nm

RRID

AB_2939948

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