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Anti-GFAP Antibody

Our Anti-GFAP mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N206A/8. It is KO validated, detects drosophila, guinea pig, human, mouse, polar bear, and rat GFAP, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Product Specifications

Background

Glial fibrillary acidic protein (GFAP) is an intermediate filament with many different isoforms, and acts as a structural protein in the cytoskeleton. It is expressed in astrocytes in brain and is often used as an astrocyte marker. GFAP is belived to be involved in many CNS processes including cell communication and mitosis. GFAP has been found to be involved in Alexander disease and Wernicke's encephalopathy.

Short Description

Our Anti-GFAP mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N206A/8. It is KO validated, detects drosophila, guinea pig, human, mouse, polar bear, and rat GFAP, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, ELISA.

Synonyms

Glial fibrillary acidic protein (GFAP)

Gene Name

GFAP

UniProt

P14136

Host

Mouse

Reactivity

Drosophila, Guinea Pig, Human, Mouse, Polar Bear, and Rat

Immunogen

Synthetic peptide amino acids 411-422 (KTVEMRDGEVIK) of human GFAP (accession number P14136)

Target

GFAP

Clonality

Monoclonal

Isotype

IgG1

Clone

N206A/8

Type

Primary Antibody

Applications

WB, IHC, ICC, ELISA

Field of Research

Glial and Schwann Cell Markers/Astrocyte Markers

Concentration

1 mg/mL

Format

Purified by Protein A chromatography

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Molecular Weight

50 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

IHC Dilution

1:500

WB Dilution

1:1000

Specificity

Cross-reacts with GFAP-R416W and other GFAP mutant proteinsDoes not cross-react with other proteins (based on KO validation results)

ICC Dilution

1:500

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Human

WB Brain Quality Control

1

Trial Size

1

Conjugate

Unconjugated

RRID

AB_10672299

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