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Anti-RBM17/SPF45 Antibody FL594 Conjugate

Our Anti-RBM17/SPF45 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N219/5. It detects human, mouse, and rat RBM17/SPF45, and is purified by Protein A chromatography. It is great for use in IHC, ICC.

Product Specifications

Background

RNA-Binding Motif Protein 17 or Splicing factor 45 is encoded by the gene RBM17. RBM17 is a component of the spliceosome complex and displays catalytic activity during mRNA splicing. RBM17 is involved in alternative splicing, resulting in different isoforms of a gene. RBM is also thought to play a role in DNA repair (Red: Chaouki et al 2006) RBM17 is widely expressed in the nucleus and important for development. Diseases associated with this gene include sickle cell anemia and forms of Ataxia 1.

Short Description

Our Anti-RBM17/SPF45 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N219/5. It detects human, mouse, and rat RBM17/SPF45, and is purified by protein a chromatography. It is great for use in IHC, ICC.

Synonyms

Splicing factor 45 (45 kDa-splicing factor) (RNA-binding motif protein 17)

Gene Name

RBM17 SPF45

UniProt

Q96I25

Host

Mouse

Reactivity

Human, Mouse, and Rat

Immunogen

Fusion protein amino acids 100-200 of human RBM17 (accession number Q96I25) produced recombinantly in E. Coli

Target

RBM17/SPF45

Clonality

Monoclonal

Isotype

IgG1

Clone

N219/5

Type

Primary Antibody

Applications

IHC, ICC

Field of Research

Cell Signaling/Neurogenesis and Development

Concentration

0.5 mg/mL

Format

Purified by Protein A chromatography

Buffer

PBS with 0.09% azide

Molecular Weight

45-52 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and conjugation of purified mAb. Purified mAbs are >90% specific antibody.

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Human

WB Brain Quality Control

1

Conjugate

FL594 Ex: 594 nm, Em: 615 nm

RRID

AB_2939854

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