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Anti-Iduna/RNF146 Antibody FL650 Conjugate

Our Anti-Iduna/RNF146 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N201/35. It detects mouse and rat Iduna/RNF146, and is purified by Protein A chromatography. It is great for use in ICC.

Product Specifications

Background

E3 ubiquitin-protein ligase RNF146, RING finger protein 146 or Iduna is encoded by the gene RNF146. Iduna is a ubiquitin protein ligase that is involved in ubiquitination and degradation. It is thought to be involved with diverse processes including DNA repair, activation of the Wnt signaling pathway, protection from DNA damage induced cell death. Iduna is found in many tissues including brain, heart, spleen, kidney and liver.

Short Description

Our Anti-Iduna/RNF146 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N201/35. It detects mouse and rat Iduna/RNF146, and is purified by protein a chromatography. It is great for use in ICC.

Synonyms

E3 ubiquitin-protein ligase RNF146 (EC 2.3.2.27) (Iduna) (RING finger protein 146) (RING-type E3 ubiquitin transferase RNF146)

Gene Name

Rnf146

UniProt

Q9CZW6

Host

Mouse

Reactivity

Mouse and Rat

Immunogen

Fusion protein amino acids 1-359 (full-length) of mouse Iduna (accession number Q9CZW6) produced recombinantly in E. Coli

Target

Iduna/RNF146

Clonality

Monoclonal

Isotype

IgG1

Clone

N201/35

Type

Primary Antibody

Applications

ICC

Field of Research

Cell Signaling/Wnt Signaling

Concentration

0.5 mg/mL

Format

Purified by Protein A chromatography

Buffer

PBS with 0.09% azide

Molecular Weight

40 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and conjugation of purified mAb. Purified mAbs are >90% specific antibody.

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Mouse

WB Brain Quality Control

1

Conjugate

FL650 Ex: 655 nm, Em: 676 nm

RRID

AB_2939851

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