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Anti-Doc2B Antibody FL650 Conjugate

Our Anti-Doc2b mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N150/21. It detects mouse and rat Doc2b, and is purified by Protein A chromatography. It is great for use in IHC, ICC.

Product Specifications

Background

Double C2-like domain-containing protein beta or Doc2b is encoded by the gene DOC2b. Doc2b functions as a calcium sensor which positively regulates soluble NSF attachment receptor (SNARE) dependent fusion of vesicles with membranes. Doc2b binds phospholipids and may act at the priming stage of membrane fusion by modifying membrane curvature to stimulate fusion. Doc2b is expressed in pancreatic cells, endocrine cells and brain, where it is highly expressed in neurons. (Friedrich et al.Neurosci., July 2, 2008 • 28 (27) :6794 – 6806)

Short Description

Our Anti-Doc2b mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N150/21. It detects mouse and rat Doc2b, and is purified by protein a chromatography. It is great for use in IHC, ICC.

Synonyms

Double C2-like domain-containing protein beta (Doc2-beta)

Gene Name

Doc2b

UniProt

P70610

Host

Mouse

Reactivity

Mouse and Rat

Immunogen

Fusion protein amino acids 1-216 (N-terminus) of rat Doc2b (accession number P70610) produced recombinantly in E. Coli

Target

Doc2b

Clonality

Monoclonal

Isotype

IgG1

Clone

N150/21

Type

Primary Antibody

Applications

IHC, ICC

Field of Research

Vesicle Trafficking/Fusion

Concentration

0.5 mg/mL

Format

Purified by Protein A chromatography

Buffer

PBS with 0.09% azide

Molecular Weight

45 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and conjugation of purified mAb. Purified mAbs are >90% specific antibody.

Specificity

Does not cross-react with Doc2a

Quality Control

Each new lot of antibody is quality control tested by IHC on either rat or mouse brain and confirmed to give the expected staining pattern.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Rat

Conjugate

FL650 Ex: 655 nm, Em: 676 nm

IHC Quality Control

1

RRID

AB_2939795

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