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Anti-LAR/PTPRF (Receptor-Type Tyrosine-Protein Phosphatase) Antibody

Our Anti-LAR/PTPRF (Receptor-type tyrosine-protein phosphatase) mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N165/38. It detects human, mouse, and rat LAR/PTPRF (Receptor-type tyrosine-protein phosphatase), and is purified by Protein A chromatography. It is great for use in ICC, WB.

Product Specifications

Background

Receptor-type tyrosine-protein phosphatase F or Leukocyte Common Antigen Related protein is encoded by the gene PTPRF and is a member of the protein tyrosine phosphatase (PTP) family. PRPRF is a transmembrane protein involved in regulation of insulin signaling, cell proliferation and cell migration. PTPRF functions in the regulation of epithelial cell-cell contacts at adherens junctions, as well as in the control of beta-catenin signaling. PTPRF is ubiquitously expressed. Diseases associated with PTPRF include Aplasia or hypoplasia of the breasts and/or nipples 2

Short Description

Our Anti-LAR/PTPRF (Receptor-type tyrosine-protein phosphatase) mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N165/38. It detects human, mouse, and rat LAR/PTPRF (Receptor-type tyrosine-protein phosphatase), and is purified by protein a chromatography. It is great for use in WB, ICC, ELISA.

Synonyms

Receptor-type tyrosine-protein phosphatase F (EC 3.1.3.48) (Leukocyte common antigen related) (LAR)

Gene Name

PTPRF LAR

UniProt

P10586

Host

Mouse

Reactivity

Human, Mouse, and Rat

Immunogen

Fusion protein amino acids 1315-1607 (cytoplasmic C-terminus) of human LAR (accession number P10586) produced recombinantly in E. Coli

Target

LAR/PTPRF (Receptor-type tyrosine-protein phosphatase)

Clonality

Monoclonal

Isotype

IgG2a

Clone

N165/38

Type

Primary Antibody

Applications

WB, ICC, ELISA

Field of Research

Cell Adhesion Molecules

Concentration

1 mg/mL

Format

Purified by Protein A chromatography

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.121

Molecular Weight

85 kDa (full-length is 210 kDa, smaller due to proteolysis into P-subunit containing transmembrane and intracellular domains)

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Specificity

Does not cross-react with PTPRD or PTPRS

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Human

WB Brain Quality Control

1

Conjugate

Unconjugated

RRID

AB_10675291

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