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Anti-6xHis Antibody FL650 Conjugate

Our Anti-6xHis mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N144/14. It detects 6xHis, and is purified by Protein A chromatography. It is great for use in ICC.

Product Specifications

Background

6xHIS or His-tag is a commonly used recombinant tag that consists of 6 sequential histidine residues placed at the amino or carboxy terminus of a targeted sequence. 6xHis is useful for purification of proteins or visualization by western blot or immunocytochemistry.

Short Description

Our Anti-6xHis mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N144/14. It detects n/a 6xHis, and is purified by protein a chromatography. It is great for use in ICC.

Host

Mouse

Immunogen

Identified as off-target mAb in screen for anti-ProtocadherinGammaA3 Neuromabs (immunogen was 6xHis fusion protein amino acids 720-804, variable portion of cytoplasmic C-terminus, of mouse ProtocadherinGammaA3) . Target identified as 6xHis by ELISA, immunofluorescence and immunoblot against other 6xHis-tagged fusion proteins.

Target

6xHis

Clonality

Monoclonal

Isotype

IgG1

Clone

N144/14

Type

Primary Antibody

Applications

ICC

Field of Research

Anti-Tag Antibodies

Concentration

0.5 mg/mL

Format

Purified by Protein A chromatography

Buffer

PBS with 0.09% azide

Molecular Weight

1 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and conjugation of purified mAb. Purified mAbs are >90% specific antibody.

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Mouse

ICC Quality Control

1

Conjugate

FL650 Ex: 655 nm, Em: 676 nm

RRID

AB_2939623

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