Anti-SMRT Antibody

Mouse Monoclonal Antibody specific to Smrt

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

N-CoR2, CTG repeat protein 26, SMAP270, Silencing mediator of retinoic acid and thyroid hormone receptor, SMRT, T3 receptor-associating factor, TRAC, Thyroid-, retinoic-acid-receptor-associated corepressor

Gene Name

NCOR2

NCBI Gene ID

UniProt

Q9Y618

Cellular Locus

Nucleus.

Host

Mouse

Reactivity

Human

Immunogen

A KLH-conjugated synthetic peptide derived from human SMRT corresponding to aa 994-1005

Target Antigen

Nuclear receptor corepressor 2

Target

SMRT

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, ICC/IF, IP

Field of Research

Neuroscience

Purification Method

Purified by immunoaffinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Transcriptional corepressor (PubMed:20812024). Mediates the transcriptional repression activity of some nuclear receptors by promoting chromatin condensation, thus preventing access of the basal transcription. Isoform 1 and isoform 4 have different affinities for different nuclear receptors. Involved in the regulation BCL6-dependent of the germinal center (GC) reactions, mainly through the control of the GC B-cells proliferation and survival. Recruited by ZBTB7A to the androgen response elements/ARE on target genes, negatively regulates androgen receptor signaling and androgen-induced cell proliferation (PubMed:20812024). {PubMed:18212045, PubMed:20812024, PubMed:23911289}.

Additionnal Information

Immunoblotting and <br><br>Immunoprecipitation: use at 1-5 ug/mL. <br><br>Positive control: HBL100

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.

Specificity

This antibody recognizes human SMRT.

Formulation

10 mM PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

SMRT (Silencing Mediator for Retinoid and Thyroid receptors) is a 168 kD nuclear protein. Nuclear hormone receptors are transcription factors regulated by ligand binding. In the absence of ligand, there is a physical interaction between the nuclear receptors that do not interact with Hsp90 and a family of corepressors which includes SMRT. SMRT helps target histone deacetylases and other components of a multiprotein repressor complex to the specific target promoter to silence gene transcription.