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Anti-Mitofusin-1 Antibody

Our Anti-Mitofusin-1 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N111/24. It is KO validated, detects mouse and rat Mitofusin-1, and is purified by Protein A chromatography. It is great for use in ICC, WB.

Product Specifications

Background

Mitofusin 1, or transmembrane GTPase Mfn1 (MFN1) is a mitochondrial membrane protein with GTPase activity that functions to aid mitochondrial clustering and fusion. It may also play a role in mitochondrial metabolism. It is expressed in the outer membrane of mitochondria in many tissues, including heart and testis. Diseases associated with MFN1 include Optic Atrophy 1 and Autosomal Recessive Spastic Paraplegia .

Short Description

Our Anti-Mitofusin-1 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N111/24. It is KO validated, detects mouse and rat Mitofusin-1, and is purified by protein a chromatography. It is great for use in WB, ICC, ELISA.

Synonyms

Mitofusin-1 (EC 3.6.5.-) (Transmembrane GTPase MFN1)

Gene Name

Mfn1

UniProt

Q811U4

Host

Mouse

Reactivity

Mouse and Rat

Immunogen

Fusion protein amino acids 350-580 of mouse Mitofusin-1 (accession number Q811U4) produced recombinantly in E. Coli

Target

Mitofusin-1

Clonality

Monoclonal

Isotype

IgG1

Clone

N111/24

Type

Primary Antibody

Applications

WB, ICC, ELISA

Field of Research

Mitochondrial Biology/Neurodegenerative Disease/Parkinson's Disease

Concentration

1 mg/mL

Format

Purified by Protein A chromatography

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.88

Molecular Weight

80 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Specificity

No cross-reactivity against Mitofusin-2

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Mouse

ICC Quality Control

1

Conjugate

Unconjugated

RRID

AB_2266317

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