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Anti-Pan-SAPAP Antibody

Our Anti-Pan-SAPAP mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N127/31. It detects human, mouse, and rat Pan-SAPAP, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Product Specifications

Background

SAPAP1 (also known as disks large associated protein 1), SAPAP2 (also known as disks large associated protein 2), SAPAP3 (also known as disks large associated protein 3) and SAPAP4 (also known as disks large associated protein 4) proteins are members of the SAPAP or DLGAP family of proteins. SAPAP proteins function as scaffolding proteins at excitatory synapses. SAPAP proteins interact with PSD95 family and Shank family proteins to anchor/target neurotransmitter receptors and signaling molecules to the postsynaptic membrane of excitatory synapses.

Short Description

Our Anti-Pan-SAPAP mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N127/31. It detects human, mouse, and rat Pan-SAPAP, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, ELISA.

Synonyms

Disks large-associated protein 1 (SAPAP1) SAPAP-1 Disks large-associated protein 2 (SAPAP2) SAPAP-2 Disks large-associated protein 3 (SAPAP3) SAPAP-3 Disks large-associated protein 4 (SAPAP-4) SAPAP4

Gene Name

DLGAP1 DAP1 GKAP DLGAP2 C8orf68 DAP2 ERICH1-AS1 DLGAP3 DAP3 DLGAP4 DAP4 KIAA0964 SAPAP4

UniProt

P97836

Host

Mouse

Reactivity

Human, Mouse, and Rat

Immunogen

Fusion protein amino acids 772-992 (C-terminus) of rat SAPAP1 (accession number P97836) produced recombinantly in E. Coli

Target

Pan-SAPAP

Clonality

Monoclonal

Isotype

IgG2b

Clone

N127/31

Type

Primary Antibody

Applications

WB, IHC, ICC, ELISA

Field of Research

Scaffold Proteins

Concentration

1 mg/mL

Format

Purified by Protein A chromatography

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.82

Molecular Weight

120 kDa (SAPAP2), 110 kDa (SAPAP1/3/4)

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

IHC Dilution

1:500

WB Dilution

1:1000

Specificity

Cross-reacts with other SAPAPs

ICC Dilution

1:500

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Rat

WB Brain Quality Control

1

Conjugate

Unconjugated

RRID

AB_10671947

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