Anti-HCN4 Antibody

Our Anti-HCN4 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N114/10. It detects human, mouse, and rat HCN4, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Product Specifications

Background

HCN4 is a member of the hyperpolarization-activated cyclic nucleotide gated channel family, also known as pacemaker channels. This family has a role in rhythmic activity of the heart and firing neurons. HCN channels are made up of four subunits, HCN1-4 and can be formed in multiple combinations. HCN4 is found in the cell membrane as a multi pass membrane protein in the sino-atrial node of the heart. This protein is highly expressed in neurons in the thalamus and other brain regions and testis. Mutations in HCN4 have been found in several types of sinus bradycardia.

Short Description

Our Anti-HCN4 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N114/10. It detects gerbil, human, mouse, pig, and rat HCN4, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, ELISA.

Synonyms

Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4

Gene Name

HCN4

UniProt

Q9Y3Q4

Host

Mouse

Reactivity

Gerbil, Human, Mouse, Pig, and Rat

Immunogen

Fusion protein amino acids 1019-1108 (cytoplasmic C-terminus) of rat HCN4 (accession number Q9JKA7) produced recombinantly in E. Coli

Target

HCN4

Clonality

Monoclonal

Isotype

IgG1

Clone

N114/10

Type

Primary Antibody

Applications

WB, IHC, ICC, ELISA

Field of Research

Ion Channels and Modulators/Other Ion Channels

Concentration

1 mg/mL

Format

Purified by Protein A chromatography

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.77

Molecular Weight

130 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

IHC Dilution

1:200

WB Dilution

1:500

Specificity

No cross-reactivity against other HCN’s

ICC Dilution

1:200

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.