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Anti-Ankyrin-G (Staining) Antibody

Our Anti-Ankyrin-G (Staining) mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N106/65. It detects human, mouse, and rat Ankyrin-G (Staining), and is purified by Protein A chromatography. It is great for use in IHC, ICC.

Product Specifications

Background

Ankyrin-G (ankyrin 3) is a member of the Ankyrin family which consists of Ankyrin R (1), Ankyrin B (2) and Ankyrin G (3) all of which are expressed in the nervous system. Ankyrins are structural proteins that aid in the attachment of integral membrane proteins with the spectrin-actin cytoskeleton, and thus targets voltage-gated sodium channels, potassium channels, cell adhesion molecules and others at the nodes of Ranvier and axonal initial segments (AIS) . Variants in Ankyrin G have been linked to bipolar disorder.

Short Description

Our Anti-Ankyrin-G (Staining) mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N106/65. It detects human, mouse, and rat Ankyrin-G (Staining), and is purified by protein a chromatography. It is great for use in IHC, ICC, ELISA, EM.

Synonyms

Ankyrin-3 (ANK-3) (Ankyrin-G)

Gene Name

ANK3

UniProt

E9PE32

Host

Mouse

Reactivity

Human, Mouse, and Rat

Immunogen

Fusion protein ~1000 amino acids of Ankyrin-G (accession number E9PE32) produced recombinantly in E. Coli

Target

Ankyrin-G (Staining)

Clonality

Monoclonal

Isotype

IgG2b

Clone

N106/65

Type

Primary Antibody

Applications

IHC, ICC, ELISA, EM

Field of Research

Axonal Markers/Scaffold Proteins

Concentration

1 mg/mL

Format

Purified by Protein A chromatography

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.74

Molecular Weight

<200 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

IHC Dilution

1:500

Specificity

Does not cross-react with Ankyrin-B

ICC Dilution

1:500

Quality Control

Each new lot of antibody is quality control tested by IHC on either rat or mouse brain and confirmed to give the expected staining pattern.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Human

Conjugate

Unconjugated

IHC Quality Control

1

RRID

AB_10675130

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