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Anti-Ankyrin-B Antibody

Our Anti-Ankyrin-B mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N105/13. It detects human, mouse, and rat Ankyrin-B, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Product Specifications

Background

Ankyrin-2 or -Ankyrin-B is encoded by the gene ANK2. Ankyrin B is a scaffold protein that functions to link proteins that mediate the attachment of integral membrane proteins to the spectrin-actin based membrane cytoskeleton. In this manner, ankyrin family proteins play key roles in various processes including cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains. Ankyrin B is expressed in brain and muscle. Mutations in this gene cause long QT syndrome 4 and cardiac arrhythmia syndrome.

Short Description

Our Anti-Ankyrin-B mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N105/13. It detects bovine, guinea pig, human, mouse, and rat Ankyrin-B, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, IP, ELISA.

Synonyms

Ankyrin-2 (ANK-2) (Ankyrin-B) (Brain ankyrin) (Non-erythroid ankyrin)

Gene Name

ANK2 ANKB

UniProt

Q01484

Host

Mouse

Reactivity

Bovine, Guinea Pig, Human, Mouse, and Rat

Immunogen

Fusion protein amino acids 203-496 of human Ankyrin-B (accession number Q01484) produced recombinantly in E. Coli

Target

Ankyrin-B

Clonality

Monoclonal

Isotype

IgG1

Clone

N105/13

Type

Primary Antibody

Applications

WB, IHC, ICC, IP, ELISA

Field of Research

Scaffold Proteins

Concentration

1 mg/mL

Format

Purified by Protein A chromatography

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.70

Molecular Weight

>200 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

IHC Dilution

1:500

WB Dilution

1:500

IP Dilution

3 ug

Specificity

No cross-reactivity reported

ICC Dilution

1:500

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Human

WB Brain Quality Control

1

Conjugate

Unconjugated

RRID

AB_10673094

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