Anti-GFP Antibody
Our Anti-GFP mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N86/8. It detects GFP, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB, ELISA, CHiP.
Product Specifications
Background
Short Description
Synonyms
Green fluorescent protein
Gene Name
GFP
UniProt
P42212
Host
Mouse
Immunogen
Fusion protein amino acids 1-238 (full-length) of jellyfish green fluorescent protein (accession number P42212) produced recombinantly in E. Coli
Target
GFP
Clonality
Monoclonal
Isotype
IgG2a
Clone
N86/8
Type
Primary Antibody
Applications
WB, IHC, ICC, IP, ELISA, ChIP
Field of Research
Anti-Tag Antibodies
Concentration
1 mg/mL
Format
Purified by Protein A chromatography
Buffer
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.57
Molecular Weight
27 kDa
Shipping Conditions
Shipped on ice packs
Storage Conditions
Notes
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
IHC Dilution
1:1000
WB Dilution
1:1000
Specificity
Cross-reacts with Cyan and Yellow fluorescent protein (CFP and YFP, respectively) Does not cross-react with Red fluorescent protein (dsRed)
ICC Dilution
1:500
Quality Control
Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.
Recommended Usage
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
Physical Properties
Liquid
Immunogen Species
Jellyfish
ICC Quality Control
1
Trial Size
1
Conjugate
Unconjugated
RRID
AB_10671445
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