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Anti-GFP Antibody FL490 Conjugate

Our Anti-GFP mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N86/8. It detects GFP, and is purified by Protein A chromatography. It is great for use in IHC, ICC.

Product Specifications

Background

Green fluorescent protein (GFP) from the jellyfish Aequorea Victoria is widely used as a marker of protein expression or targeting in cells or model organisms.

Short Description

Our Anti-GFP mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N86/8. It detects n/a GFP, and is purified by protein a chromatography. It is great for use in IHC, ICC.

Synonyms

Green fluorescent protein

Gene Name

GFP

UniProt

P42212

Host

Mouse

Immunogen

Fusion protein amino acids 1-238 (full-length) of jellyfish green fluorescent protein (accession number P42212) produced recombinantly in E. Coli

Target

GFP

Clonality

Monoclonal

Isotype

IgG2a

Clone

N86/8

Type

Primary Antibody

Applications

IHC, ICC

Field of Research

Anti-Tag Antibodies

Concentration

0.5 mg/mL

Format

Purified by Protein A chromatography

Buffer

PBS with 0.09% azide

Molecular Weight

27 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and conjugation of purified mAb. Purified mAbs are >90% specific antibody.

Specificity

Cross-reacts with Cyan and Yellow fluorescent protein (CFP and YFP, respectively) Does not cross-react with Red fluorescent protein (dsRed)

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Jellyfish

ICC Quality Control

1

Conjugate

FL490 Ex: 491 nm, Em: 515 nm

RRID

AB_2939484

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