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Anti-Mortalin/GRP75 Antibody FL650 Conjugate

Our Anti-Mortalin/GRP75 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N52A/42. It detects human, mouse, and rat Mortalin/GRP75, and is purified by Protein A chromatography. It is great for use in IHC, ICC.

Product Specifications

Background

Mitochondrial stress-70 protein or Mortalin/GRP75 is encoded by the gene HSPA9. Mortalin is a chaperone protein and plays a role in cell proliferation, stress response and mitochondria maintenance. It is widely expressed in tissues in mitochondria, endoplasmic reticulum, plasma membrane and cytoplasmic vesicles. Diseases associated with this gene include Even-Plus Syndrome and Sideroblastic Anemia 4.

Short Description

Our Anti-Mortalin/GRP75 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N52A/42. It detects human, mouse, non-human primate, and rat Mortalin/GRP75, and is purified by protein a chromatography. It is great for use in IHC, ICC.

Synonyms

Stress-70 protein, mitochondrial (75 kDa glucose-regulated protein) (GRP-75) (Heat shock 70 kDa protein 9) (Mortalin) (Peptide-binding protein 74) (PBP74) (p66 MOT)

Gene Name

Hspa9 Grp75 Hsp74 Hspa9a

UniProt

P38647

Host

Mouse

Reactivity

Human, Mouse, Non-Human Primate, and Rat

Immunogen

Identified as off-target mAb in screen for anti-Salm2 NeuroMabs (immunogen was fusion protein amino acids 551-766 of mouse SALM2) . Target identified as Mortalin/Grp75 by LC-MS/MS analysis of protein immunopurified from rat brain using N52A/42.

Target

Mortalin/GRP75

Clonality

Monoclonal

Isotype

IgG1

Clone

N52A/42

Type

Primary Antibody

Applications

IHC, ICC

Field of Research

Mitochondrial Biology/Neurodegenerative Disease/Parkinson's Disease

Concentration

0.5 mg/mL

Format

Purified by Protein A chromatography

Buffer

PBS with 0.09% azide

Molecular Weight

75 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and conjugation of purified mAb. Purified mAbs are >90% specific antibody.

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Mouse

WB Brain Quality Control

1

Conjugate

FL650 Ex: 655 nm, Em: 676 nm

RRID

AB_2939475

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