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Anti-HCN2 Antibody FL650 Conjugate

Our Anti-HCN2 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N71/37. It detects mouse and rat HCN2, and is purified by Protein A chromatography. It is great for use in IHC, ICC.

Product Specifications

Background

HCN2 is a member of the hyperpolarization-activated cyclic nucleotide gated channel family, also known as pacemaker channels. This family has a role in rhythmic activity of the heart and firing neurons. HCN channels are made up of four subunits, HCN1-4 and can be formed in multiple combinations. HCN2 is expressed throughout the brain and in high levels in thalamic and brainstem nuclei.

Short Description

Our Anti-HCN2 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N71/37. It detects human, mouse, and rat HCN2, and is purified by protein a chromatography. It is great for use in IHC, ICC.

Synonyms

Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2

Gene Name

Hcn2

UniProt

Q9JKA9

Host

Mouse

Reactivity

Human, Mouse, and Rat

Immunogen

Fusion protein amino acids 761-863 (cytoplasmic C-terminus) of rat HCN2 (accession number Q9JKA9) produced recombinantly in E. Coli

Target

HCN2

Clonality

Monoclonal

Isotype

IgG1

Clone

N71/37

Type

Primary Antibody

Applications

IHC, ICC

Field of Research

Ion Channels and Modulators/Other Ion Channels

Concentration

0.5 mg/mL

Format

Purified by Protein A chromatography

Buffer

PBS with 0.09% azide

Molecular Weight

95 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and conjugation of purified mAb. Purified mAbs are >90% specific antibody.

Specificity

No cross-reactivity against HCN1

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Rat

WB Brain Quality Control

1

Conjugate

FL650 Ex: 655 nm, Em: 676 nm

RRID

AB_2939419

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