What is the catalog number for Anti-HCN1 Antibody?

The catalog number for Anti-HCN1 Antibody is 75-110-020.

What sizes are available for Anti-HCN1 Antibody?

Anti-HCN1 Antibody is available in the following size(s): 20 µL.

Is Anti-HCN1 Antibody in stock?

Anti-HCN1 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Anti-HCN1 Antibody

Our Anti-HCN1 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N70/28. It is KO validated, detects human, mouse, and rat HCN1, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.

Product Specifications

Background

HCN1 is a member of the hyperpolarization-activated cyclic nucleotide gated channel family, also known as pacemaker channels. This family has a role in rhythmic activity of the heart and firing neurons. HCN channels are made up of four subunits, HCN1-4 and can be formed in multiple combinations. HCN1 is found in the hippocampus, neocortex, and cerebellar cortex of brain. Alterations in this protein have been implicated in the disease early infantile epileptic encephalopathy.

Short Description

Our Anti-HCN1 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N70/28. It is KO validated, detects gerbil, human, non-human primate, mouse, and rat HCN1, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, IP, ELISA, EM.

Synonyms

Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1

Gene Name

Hcn1

UniProt

Q9JKB0

Host

Mouse

Reactivity

Gerbil, Human, Non-Human Primate, Mouse, and Rat

Immunogen

Fusion protein amino acids 778-910 (cytoplasmic C-terminus) of rat HCN1 (accession number Q9JKB0) ; Mouse: 96% identity (128/133 amino acids identical) produced recombinantly in E. Coli

Target

HCN1

Clonality

Monoclonal

Isotype

IgG1

Clone

N70/28

Type

Primary Antibody

Applications

WB, IHC, ICC, IP, ELISA, EM

Field of Research

Ion Channels and Modulators/Other Ion Channels

Concentration

1 mg/mL

Format

Purified by Protein A chromatography

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.38

Molecular Weight

100 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

IHC Dilution

1:500

WB Dilution

1:500

IP Dilution

10 μg

Specificity

No cross-reactivity against HCN2

ICC Dilution

1:100

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.