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Anti-Ataxin-1, WT Antibody (BSA and Azide free)

Our carrier-free Anti-Ataxin-1, WT mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N65/37. It detects human, mouse, and rat Ataxin-1, WT, and is purified by Protein A chromatography. It is great for use in ICC, WB.

Product Specifications

Background

Ataxin1, also known as spinocerebellar ataxia type 1 protein homolog, is a highly conserved DNA-binding protein. Ataxin1 is expressed in brain and can be found at high levels in the cortex and the hypothalamus in neurons. It is also expressed in other tissues. Within the cell, it is highly expressed in the nucleus, and can also be found in the cytoplasm. Mutations in the ataxin-1 gene cause spinocerebellar ataxia type 1.

Short Description

Our Anti-Ataxin-1, WT mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N65/37. It detects human, mouse, and rat Ataxin-1, WT, and is purified by protein a chromatography. It is great for use in WB, ICC, ELISA.

Synonyms

Ataxin-1 (Spinocerebellar ataxia type 1 protein homolog)

Gene Name

Atxn1 Sca1

UniProt

P54254

Host

Mouse

Reactivity

Human, Mouse, and Rat

Immunogen

Synthetic peptide amino acids 746-761 (RKRRRWSAPETRKLEK) of mouse ataxin-1 (accession number P54254)

Target

Ataxin-1, WT

Clonality

Monoclonal

Isotype

IgG1

Clone

N65/37

Type

Primary Antibody

Applications

WB, ICC, ELISA

Field of Research

Rare Diseases

Concentration

1 mg/mL

Format

Purified by Protein A chromatography

Buffer

PBS

Molecular Weight

85 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and buffer exchanged into 1X PBS. Purified mAbs are >90% specific antibody.

Specificity

Minimal cross-reactivity against S751A mutant of Ataxin-1 by ELISA and immunofluorescence and negative by immunoblot (right)

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Mouse

Conjugate

Unconjugated

IHC Quality Control

1

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