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Anti-Cav3.2 Ca2+ Channel Antibody

Our Anti-Cav3.2 Ca2+ channel mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N55/10. It is KO validated, detects human and mouse Cav3.2 Ca2+ channel, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.

Product Specifications

Background

Voltage-dependent T-type calcium channel subunit alpha-1H or Cav3.2 Ca2+ channel is encoded by the gene CACNA1H. Cav3.2 is a pore forming alpha subunit of T voltage-sensitive calcium channels and gives rise to T-type calcium currents. (transient opening calcium channels) . These channels facilitate entry of calcium ions into excitable cells are involved in a variety of calcium dependent processes, including neurotransmitter release, muscle contraction, and gene expression. Cav3.2 is expressed in kidney, liver, and heart and brain.as a membrane bound protein. Diseases associated with this gene include Hyperaldosteronism and Epilepsy.

Short Description

Our Anti-Cav3.2 Ca2+ channel mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N55/10. It is KO validated, detects human and mouse Cav3.2 Ca2+ channel, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, IP, ELISA.

Synonyms

Voltage-dependent T-type calcium channel subunit alpha-1H (Low-voltage-activated calcium channel alpha1 3.2 subunit) (Voltage-gated calcium channel subunit alpha Cav3.2)

Gene Name

CACNA1H

UniProt

O95180

Host

Mouse

Reactivity

Human and Mouse

Immunogen

Fusion protein amino acids 1019-1293 (cytoplasmic loop between repeat II and repeat III) of human Cav3.2 (accession number O95180), epitope mapped to amino acids 1179-1192 (AEDGRAAPGPRATP) produced recombinantly in E. Coli

Target

Cav3.2 Ca2+ channel

Clonality

Monoclonal

Isotype

IgG1

Clone

N55/10

Type

Primary Antibody

Applications

WB, IHC, ICC, IP, ELISA

Field of Research

Ion Channels and Modulators/Ca2+ Channels

Concentration

1 mg/mL

Format

Purified by Protein A chromatography

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.22

Molecular Weight

260 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Specificity

Does not cross-react with Cav3.1

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Human

ICC Quality Control

1

Conjugate

Unconjugated

RRID

AB_2315827

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