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Anti-NGL-2/LRRC4 Antibody

Our Anti-NGL-2/LRRC4 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N50/36. It is KO validated, detects mouse and rat NGL-2/LRRC4, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Product Specifications

Background

Leucine Rich Repeat Containing 4 is encoded by the gene LRRC4. LRRC4 is a synaptic adhesion protein that regulates the formation of excitatory synapses through the recruitment of pre and postsynaptic proteins. LRRC4 organizes the lamina pathway-specific differentiation of dendrites, and plays an important role in auditory synaptic responses. LRRC4 is expressed in the brain. LRRC4 is significantly downregulated in primary brain tumors.

Short Description

Our Anti-NGL-2/LRRC4 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N50/36. It is KO validated, detects mouse and rat NGL-2/LRRC4, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, ELISA.

Synonyms

Leucine-rich repeat-containing protein 4 (Brain tumor-associated protein MBAG1) (Netrin-G2 ligand) (NGL-2)

Gene Name

Lrrc4

UniProt

Q99PH1

Host

Mouse

Reactivity

Mouse and Rat

Immunogen

Fusion protein amino acids 550-652 of mouse NGL-2 (accession number AAG60620) produced recombinantly in E. Coli

Target

NGL-2/LRRC4

Clonality

Monoclonal

Isotype

IgG1

Clone

N50/36

Type

Primary Antibody

Applications

WB, IHC, ICC, ELISA

Field of Research

Cell Adhesion Molecules

Concentration

1 mg/mL

Format

Purified by Protein A chromatography

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.14

Molecular Weight

100 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

IHC Dilution

1:100

Specificity

No cross-reactivity against NGL-1 or NGL-3

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Mouse

WB Brain Quality Control

1

Conjugate

Unconjugated

RRID

AB_2137614

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