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Anti-ADAM22 (Cytoplasmic) Antibody

Our Anti-ADAM22 (cytoplasmic) mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N46/30. It is KO validated, detects human, mouse, and rat ADAM22 (cytoplasmic), and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.

Product Specifications

Background

ADAM22 is a member of the ADAM (a disintegrin and metalloproteinase) family and predominantly expressed in brain. ADAM 22 is non-catalytic unlike other proteins in the family as it does not contain a zinc binding domain. ADAM22 is a post synaptic neuron receptor for LGI 1 and also binds LGI4. ADAM22 serve as an integrin ligand. Mutations in ADAM22 are implicated in early infantile epileptic encephalopathy-61 and Brachydactyly type C.

Short Description

Our Anti-ADAM22 (cytoplasmic) mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N46/30. It is KO validated, detects human, mouse, and rat ADAM22 (cytoplasmic), and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, IP, ELISA.

Synonyms

Disintegrin and metalloproteinase domain-containing protein 22 (ADAM 22)

Gene Name

Adam22

UniProt

Q9R1V6

Host

Mouse

Reactivity

Human, Mouse, and Rat

Immunogen

Fusion protein amino acids 757-857 (cytoplasmic region) of mouse ADAM22 (accession number Q9R1V6) produced recombinantly in E. Coli

Target

ADAM22 (cytoplasmic)

Clonality

Monoclonal

Isotype

IgG2b

Clone

N46/30

Type

Primary Antibody

Applications

WB, IHC, ICC, IP, ELISA

Field of Research

Cell Adhesion Molecules

Concentration

1 mg/mL

Format

Purified by Protein A chromatography

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.13

Molecular Weight

90 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

IHC Dilution

1:100

WB Dilution

1:500

IP Dilution

1:250

Specificity

No cross-reactivity reported

ICC Dilution

1:100

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Mouse

WB Brain Quality Control

1

Conjugate

Unconjugated

RRID

AB_10675128

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