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Anti-CASPR2 Antibody

Our Anti-CASPR2 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone K67/25. It is KO validated, detects human, mouse, and rat CASPR2, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.

Product Specifications

Background

Contactin-associated protein-like 2 protein, or Caspr2, is a neuronal transmembrane member of the Neurexin family. Structural features of Caspr2 include epidermal growth factor repeats, laminin G domains, F5/8 type C domain, fibrinogen-like domains, and PDZ binding sites. Caspr2 is a cell adhesion molecule as well as a receptor. It is involved in mediating cell–cell interactions in the nervous system and involved in neural-glia interactions and clustering of potassium channels in myelinated axons. It is expressed at the juxaperanodal region of nodes of Ranvier in the CNS and PNS. Changes in expression have been associated with increased susceptibility to autism and epilepsy.

Short Description

Our Anti-CASPR2 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone K67/25. It is KO validated, detects human, mouse, and rat CASPR2, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, IP, ELISA.

Synonyms

Contactin associated protein-like 2 (Contactin associated protein-like 2, isoform CRA_b)

Gene Name

CNTNAP2 hCG_15499 tcag7.496

UniProt

A0A090N7T7

Host

Mouse

Reactivity

Human, Mouse, and Rat

Immunogen

Fusion protein 96-1265 (extracellular domain) of human Caspr2 (accession number A0A090N7T7) produced recombinantly in E. Coli

Target

CASPR2

Clonality

Monoclonal

Isotype

IgG2a

Clone

K67/25

Type

Primary Antibody

Applications

WB, IHC, ICC, IP, ELISA

Field of Research

Cell Adhesion Molecules

Concentration

1 mg/mL

Format

Purified by Protein A chromatography

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.7

Molecular Weight

180 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

IHC Dilution

1:100

WB Dilution

1:500

Specificity

No cross-reactivity against Caspr/paranodin

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Human

WB Brain Quality Control

1

Conjugate

Unconjugated

RRID

AB_2245198

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