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Anti-VGlut3 Antibody

Our Anti-VGlut3 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N34/34. It detects electric ray, human, mouse and rat VGlut3, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Product Specifications

Background

Vesicular glutamate transporter 3 (VGLUT3) or Solute Carrier Family 17 Member 8 is encoded by the gene SLC17A8 and belongs to the vesicular glutamate transporter family. Vglut3 transports glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. Vglut3 is expressed in many tissues including brain, kidney and liver. In brain, it is expressed within glutamatergic neurons in the amygdala, brainstem, cerebral cortex, dorsal root ganglia, dorsal spinal cord, hippocampus, and hypothalamus. Diseases associated with SLC17A8 include Deafness.

Short Description

Our Anti-VGlut3 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N34/34. It detects electric ray, human, mouse and rat VGlut3, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, ELISA.

Synonyms

Vesicular glutamate transporter 3 (VGluT3) (Solute carrier family 17 member 8)

Gene Name

Slc17a8 Vglut3

UniProt

Q7TSF2

Host

Mouse

Reactivity

Electric Ray, Human, Mouse and Rat

Immunogen

Fusion protein amino acids 546-588 (cytoplasmic C-terminus) of rat VGlut3 (accession number NP_714947) produced recombinantly in E. Coli

Target

VGlut3

Clonality

Monoclonal

Isotype

IgG1

Clone

N34/34

Type

Primary Antibody

Applications

WB, IHC, ICC, ELISA

Concentration

1 mg/mL

Format

Purified by Protein A chromatography

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.6

Molecular Weight

65 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

IHC Dilution

1:250

WB Dilution

1:100

Specificity

No cross-reactivity reported

ICC Dilution

1:200

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Rat

ICC Quality Control

1

Conjugate

Unconjugated

RRID

AB_2187711

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