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Anti-VGlut2 Antibody

Our Anti-VGlut2 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N29/29. It detects electric ray, human, mouse and rat VGlut2, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Product Specifications

Background

Vesicular glutamate transporter 2 (Vglut2) is encoded by the gene Slc17a6 and belongs to the vesicular glutamate transporter family. Vglut2 is expressed in the thalamus, midbrain, brainstem, and spinal cord at synaptic vesicles. It functions to uptake glutamate into synaptic vesicles in excitatory neural cells. Altered expression of Vglut2 has been implicate in include Gnathodiaphyseal Dysplasia and tendinosis.

Short Description

Our Anti-VGlut2 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N29/29. It detects electric ray, human, mouse and rat VGlut2, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, ELISA.

Synonyms

Vesicular glutamate transporter 2 (VGluT2) (Differentiation-associated BNPI) (Differentiation-associated Na (+) -dependent inorganic phosphate cotransporter) (Solute carrier family 17 member 6)

Gene Name

Slc17a6 Dnpi Vglut2

UniProt

Q9JI12

Host

Mouse

Reactivity

Electric Ray, Human, Mouse and Rat

Immunogen

Fusion protein amino acids 501-582 (cytoplasmic C-terminus) of rat VGlut2 (accession number Q9JI12) produced recombinantly in E. Coli

Target

VGlut2

Clonality

Monoclonal

Isotype

IgG1

Clone

N29/29

Type

Primary Antibody

Applications

WB, IHC, ICC, ELISA

Field of Research

Transporter Proteins

Concentration

1 mg/mL

Format

Purified by Protein A chromatography

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.4

Molecular Weight

60 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

IHC Dilution

1:1000

WB Dilution

1:400

Specificity

Does not cross-react with VGlut1 or VGlut3

ICC Dilution

1:1000

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Rat

WB Brain Quality Control

1

Conjugate

Unconjugated

RRID

AB_2239153

Frequently Asked Questions

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