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Anti-VGlut1 Antibody (BSA and Azide free)

Our carrier-free Anti-VGlut1 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N28/9. It detects fish, human, mouse, and rat VGlut1, and is purified by Protein A chromatography. It is great for use in Flow, IHC, ICC, WB.

Product Specifications

Background

Vesicular glutamate transporter 1 (Vglut1) is encoded by the gene Slc17a7 and belongs to the vesicular glutamate transporter family. Vglut1 is expressed in glutamatergic neurons in brain regions including the cerebellum, cerebral cortex and hippocampus at synaptic vesicles. Isoform 2 is expressed in retinal. Vglut1 functions to uptake glutamate into synaptic vesicles in excitatory neural cells. Altered expression of Vglut1 has been implicated in several neurological and psychiatric diseases including Alzheimer’s disease, Parkinson’s disease, epilepsy and schizophrenia.

Short Description

Our Anti-VGlut1 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N28/9. It detects fish, human, mouse, and rat VGlut1, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, ELISA.

Synonyms

Vesicular glutamate transporter 1 (VGluT1) (Brain-specific Na (+) -dependent inorganic phosphate cotransporter) (Solute carrier family 17 member 7)

Gene Name

Slc17a7 Bnpi Vglut1

UniProt

Q62634

Host

Mouse

Reactivity

Fish, Human, Mouse, and Rat

Immunogen

Fusion protein amino acids 493-560 (cytoplasmic C-terminus) of rat VGlut1 (accession number Q62634) produced recombinantly in E. Coli

Target

VGlut1

Clonality

Monoclonal

Isotype

IgG1

Clone

N28/9

Type

Primary Antibody

Applications

WB, IHC, ICC, ELISA

Concentration

1 mg/mL

Format

Purified by Protein A chromatography

Buffer

PBS

Molecular Weight

52 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and buffer exchanged into 1X PBS. Purified mAbs are >90% specific antibody.

Specificity

Does not cross-react with VGlut2 or VGlut3

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Rat

WB Brain Quality Control

1

Conjugate

Unconjugated

Frequently Asked Questions

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