Anti-Shank1 Antibody FL490 Conjugate

SH3 and multiple ankyrin repeat domains protein 1, or shank 1 is a member of the shank family. Shank 1 binding domains include a SH3 domain, a proline rich domain, a PDZ domain and ankyrin repeat domains. Unlike other shank family proteins, shank 1 is found in only in brain in the neuropil of cortex, CA1 region hippocampus and molecular layer of cerebellum, amygdala, substantia nigra and thalamus. Cellularly, shank 1 can be found in cytoplasm, cell junctions, postsynaptic cell membrane and postsynaptic density. Many isoforms of shank 1 exist, and some are expressed during development, while others are expressed in mature tissue. Shank 1 acts as a scaffold protein anchoring and connecting membrane bound receptors and other proteins to the cytoskeleton in neurons to enable cell signaling. In this manner, Shank 1 may play a role in synapse formation and dendritic spine maturation. Mutations in this gene have been associated with autism spectrm disorder and diabetic encephalopathy.

Our Anti-Shank1 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N22/21. It detects human, mouse, and rat Shank1, and is purified by protein a chromatography. It is great for use in IHC, ICC.

SH3 and multiple ankyrin repeat domains protein 1 (Shank1) (GKAP/SAPAP-interacting protein) (SPANK-1) (Somatostatin receptor-interacting protein) (SSTR-interacting protein) (SSTRIP) (Synamon)

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and conjugation of purified mAb. Purified mAbs are >90% specific antibody.

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.