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Anti-Cav1.2 Ca2+ Channel Antibody

Our Anti-Cav1.2 Ca2+ channel mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone L57/46. It detects guinea pig, human, mouse, and rat Cav1.2 Ca2+ channel, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.

Product Specifications

Background

Voltage-dependent L-type calcium channel subunit alpha-1C or Cav1.2 calcium channel (other names include as CACNA1C, CACH2, CACN2, CACNL1A1, CCHL1A1) is a calcium channel encoded by the gene CACNA1C. It is a member of the L type voltage dependent calcium channel family. These calcium channels mediate the influx of calcium ions into a cell upon membrane polarization. Cav1.2 is expressed in many tissues including smooth muscle, liver, kidney brain and heart. In brain, it can be detected in the hippocampus and brain cortex in the post-synaptic density and in neuronal cell bodies. Calcium channels are involved in many cell processes including muscle contraction, neurotransmitter release, gene expression, cell division and cell death. Mutations in the Cav1.2 gene have been associated with Timothy syndrome, Brugada syndrome 3 and Long QT syndrome 8.

Short Description

Our Anti-Cav1.2 Ca2+ channel mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone L57/46. It detects guinea pig, human, mouse, and rat Cav1.2 Ca2+ channel, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, IP, ELISA.

Synonyms

Voltage-dependent L-type calcium channel subunit alpha-1C (Calcium channel, L type, alpha-1 polypeptide, isoform 1, cardiac muscle) (Smooth muscle calcium channel blocker receptor) (CACB-receptor) (Voltage-gated calcium channel subunit alpha Cav1.2)

Gene Name

CACNA1C CACH2 CACN2 CACNL1A1 CCHL1A1

UniProt

P15381

Host

Mouse

Reactivity

Guinea Pig, Human, Mouse, and Rat

Immunogen

Fusion protein amino acids 1507-1733 (intracellular carboxyl terminus) of rabbit Cav1.2 (accession number P15381) produced recombinantly in E. Coli

Target

Cav1.2 Ca2+ channel

Clonality

Monoclonal

Isotype

IgG2b

Clone

L57/46

Type

Primary Antibody

Applications

WB, IHC, ICC, IP, ELISA

Field of Research

Ion Channels and Modulators/Ca2+ Channels

Concentration

1 mg/mL

Format

Purified by Protein A chromatography

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.4

Molecular Weight

240 kDa (varies with cell background due to glycosylation)

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

IHC Dilution

1:100

WB Dilution

1:1000

IP Dilution

2 μg

Specificity

No cross-reactivity reported

ICC Dilution

1:100

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Rabbit

WB Brain Quality Control

1

Conjugate

Unconjugated

RRID

AB_10673150

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