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Anti-IP3 Receptor, Type 1 Antibody

Our Anti-IP3 receptor, type 1 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone L24/18. It detects human, mouse, and rat IP3 receptor, type 1, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.

Product Specifications

Background

Inositol 1,4,5-trisphosphate receptor type 1 or IP3 receptor type 1 (IP3R1) serves as a receptor for IP3. It is expressed in many tissues in the endoplasmic reticulum to facilitate calcium release from the ER. In brain, (IP3R1) is predominantly expressed in cerebellar Purkinje neurons.. Mutations in this gene cause spinocerebellar ataxia type 15 and spinocerebellar Ataxia 29.

Short Description

Our Anti-IP3 receptor, type 1 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone L24/18. It detects canine, human, mouse, and rat IP3 receptor, type 1, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, IP, ELISA.

Synonyms

Inositol 1,4,5-trisphosphate receptor type 1 (IP3 receptor isoform 1) (IP-3-R) (IP3R 1) (InsP3R1) (Type 1 inositol 1,4,5-trisphosphate receptor) (Type 1 InsP3 receptor)

Gene Name

Itpr1 Insp3r

UniProt

P29994

Host

Mouse

Reactivity

Canine, Human, Mouse, and Rat

Immunogen

Fusion protein amino acids 2680-2749 (cytoplasmic carboxyl terminus) of rat type 1 IP3R (accession number P29994) produced recombinantly in E. Coli

Target

IP3 receptor, type 1

Clonality

Monoclonal

Isotype

IgG1

Clone

L24/18

Type

Primary Antibody

Applications

WB, IHC, ICC, IP, ELISA

Field of Research

Ion Channels and Modulators/Other Ion Channels

Concentration

1 mg/mL

Format

Purified by Protein A chromatography

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.4

Molecular Weight

300 kDa (varies due to glycosylation)

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

IHC Dilution

1:100

WB Dilution

1:1000

Specificity

Does not recognize type 2 and type 3 IP3R’s

ICC Dilution

1:100

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Rat

WB Brain Quality Control

1

Conjugate

Unconjugated

RRID

AB_10000362

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