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Anti-VaChT Antibody

Our Anti-VAChT mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N6/38. It detects electric ray, human, mouse and rat VAChT, and is purified by Protein A chromatography. It is great for use in IHC, ICC.

Product Specifications

Background

Vesicular acetylcholine transporter (VaCht), or Solute Carrier Family 18 Member A3 is encoded by the gene Slc18a3 and is a member of the vesicular amine transporter family. VaCht transports acetylcholine into secretory vesicles for release into the extracellular space. VaCht is expressed in major cholinergic cell groups in the peripheral and central cholinergic nervous systems. Diseases associated with SLC18A3 include Myasthenic Syndrome and Presynaptic and Presynaptic Congenital Myasthenic Syndromes.

Short Description

Our Anti-VAChT mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N6/38. It detects electric ray, human, mouse and rat VAChT, and is purified by protein a chromatography. It is great for use in IHC, ICC, ELISA.

Synonyms

Vesicular acetylcholine transporter (VAChT) (rVAT) (Solute carrier family 18 member 3)

Gene Name

Slc18a3 Vacht Vat

UniProt

Q16572

Host

Mouse

Reactivity

Electric Ray, Human, Mouse and Rat

Immunogen

Synthetic peptide amino acids 521-532 (CEDDYNYYYTRS) of human VAChT (accession number Q16572)

Target

VAChT

Clonality

Monoclonal

Isotype

IgG1

Clone

N6/38

Type

Primary Antibody

Applications

IHC, ICC, ELISA

Field of Research

Transporter Proteins

Concentration

1 mg/mL

Format

Purified by Protein A chromatography

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.4

Molecular Weight

55 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

IHC Dilution

1:250

WB Dilution

1:250

Specificity

No cross-reactivity reported

ICC Dilution

1:500

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Human

ICC Quality Control

1

Conjugate

Unconjugated

RRID

AB_2188148

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