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Anti-Kv4.3 K+ Channel Antibody FL650 Conjugate

Our Anti-Kv4.3 K+ channel mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone K75/41. It is KO validated, detects drosophila, guinea pig, human, mouse, and rat Kv4.3 K+ channel, and is purified by Protein A chromatography. It is great for use in IHC, ICC.

Product Specifications

Background

Kv4.3 K+ channel (Potassium voltage-gated channel subfamily D member 3) is a member of the mammalian Shal-related family and the potassium voltage gated channel subunit family. Kv4 subunit family includes four Kv4 α subunits (Kv4.1, Kv4.2 and Kv4.3) . However only Kv4.2 and Kv4.3 are predominantely epressed in the brain (Alfaro-Ruiz et al., 2005) . KV4.3 subunit is encoded by gene KCND3 in human. It has been demonstrated that Kv 4.2 and Kv4.3 are often colocalized with pain-modulating molecules in rat spinal lamina II excitatory interneurons (Huang et al., 2005) .

Short Description

Our Anti-Kv4.3 K+ channel mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone K75/41. It is KO validated, detects drosophila, guinea pig, human, mouse, and rat Kv4.3 K+ channel, and is purified by protein a chromatography. It is great for use in IHC, ICC.

Synonyms

Potassium voltage-gated channel subfamily D member 3 (Voltage-gated potassium channel subunit Kv4.3)

Gene Name

Kcnd3

UniProt

Q62897

Host

Mouse

Reactivity

Drosophila, Guinea Pig, Human, Mouse, and Rat

Immunogen

Fusion protein amino acids 415-636 (cytoplasmic C- terminus) of rat Kv4.3 (accession number Q62897) produced recombinantly in E. Coli

Target

Kv4.3 K+ channel

Clonality

Monoclonal

Isotype

IgG1

Clone

K75/41

Type

Primary Antibody

Applications

IHC, ICC

Field of Research

Ion Channels and Modulators/K+ Channels

Concentration

0.5 mg/mL

Format

Purified by Protein A chromatography

Buffer

PBS with 0.09% azide

Molecular Weight

75 kDa in rat brain membrane preparations

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and conjugation of purified mAb. Purified mAbs are >90% specific antibody.

Specificity

No cross-reactivity against rat Kv4.2 expressed in transfected cells.

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Rat

WB Brain Quality Control

1

Conjugate

FL650 Ex: 655 nm, Em: 676 nm

RRID

AB_2939131

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