Anti-CASK Antibody

Our Anti-CASK mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone K56A/50. It is KO validated, detects chicken, human, mouse, rat, Xenopus, and zebrafish CASK, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.

Product Specifications

Background

Calcium/Calmodulin-Dependent Serine Protein Kinase or CASK scaffolding protein is encoded by the gene CASK. CASK is a member of the MAGUK (membrane-associated guanylate kinase) protein family. CASK is a a calcium/calmodulin-dependent serine protein kinase. CASK is found in many different tissues including kidney, lung, liver and brain. In the brain, CASK protein is located at synapses. CASK acts as a multidomain scaffolding protein with a role in synaptic transmembrane protein anchoring and ion channel trafficking. Mutations in this gene are associated with FG syndrome 4, microcephaly with pontine and cerebellar hypoplasia, and a form of X-linked intellectual disability.

Short Description

Our Anti-CASK mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone K56A/50. It is KO validated, detects chicken, human, mouse, rat, xenopus, and zebrafish CASK, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, IP, ELISA.

Synonyms

Peripheral plasma membrane protein CASK (hCASK) (EC 2.7.11.1) (Calcium/calmodulin-dependent serine protein kinase) (Protein lin-2 homolog)

Gene Name

CASK LIN2

UniProt

O14936

Host

Mouse

Reactivity

Chicken, Human, Mouse, Rat, Xenopus, and Zebrafish

Immunogen

Fusion protein amino acids 318-415 of rat CASK/Lin2 (accession number Q62915) produced recombinantly in E. Coli

Target

CASK

Clonality

Monoclonal

Isotype

IgG1

Clone

K56A/50

Type

Primary Antibody

Applications

WB, IHC, ICC, IP, ELISA

Field of Research

Scaffold Proteins

Concentration

1 mg/mL

Format

Purified by Protein A chromatography

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.4

Molecular Weight

100 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

IHC Dilution

1:500

WB Dilution

1:1000

Specificity

No cross-reactivity reported

ICC Dilution

1:500

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.