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Anti-Complement C3a/C3a (desArg) /C3 Antibody

Our mouse Complement C3a/C3a (desArg) /C3 monoclonal antibody from Antibodies Incorporated is produced in-house from clone K13/16-5.7. It detects human Complement C3a/C3a (desArg) /C3 and is Protein A or Protein G purified. Works in ELISA, IF, IHC, WB.

Product Specifications

Background

Complement C3a is an anaphylatoxin of 77 amino acid residues released by the action of the C3 convertases on the N-terminal of the alpha chain of C3. It is rapidly inactivated by serum carboxypeptidase N which removes the C-terminal arginine residue generating C3a (desArg) .

Synonyms

C3, AHUS5, ARMD9, ASP, C3a, C3b, CPAMD1, HEL-S-62p, complement C3, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, C3a anaphylatoxin, acylation-stimulating protein cleavage product, complement component 3, complement component C3a, complement component C3b, epididymis secretory sperm binding protein Li 62p, prepro-C3

Gene Name

C3

NCBI Gene ID

718

UniProt

P01024

Host

Mouse

Reactivity

Human

Immunogen

Human C3a

Target

Complement C3a/C3a (desArg) /C3

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

K13/16-5.7

Type

Primary Antibodies

Applications

ELISA, IF, IHC, WB

Concentration

1 mg/mL ±15%

Format

Protein A or Protein G purified

Buffer

0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide

Molecular Weight

187 kDA (Native C3), 9 kDa (C3a and C3a (desArg) )

Shipping Conditions

Shipped on ice packs

Storage Conditions

2-8°C without exposure to light

Notes

BSA free

IHC Dilution

0.14583333333333334

Specificity

GAU 013-16 is specific for human C3, C3a and C3a (desArg) (1) and does not cross-react with C4a or C5a. (2, 3) GAU 013-16 recognizes a different epitope on the 9 kDa C3a than GAU 017-01 (2, 3) . No reaction is seen with a synthetic octapeptide representing the C3a C-terminal. (2)

Brand

Antibodies Inc (via BioPorto acquisition)

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Human

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