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Anti-Complement C5b-9 Antibody

Our mouse Complement C5b-9 monoclonal antibody from Antibodies Incorporated is produced from clone aE11. It detects horse, human, non-human primate, and pig Complement C5b-9 and is Protein A or Protein G purified. Works in ELISA, IF, IHC, WB.

Product Specifications

Background

C5b-9 is also known as the terminal complement complex (TCC) . The TCC consists of C5b, C6, C7, C8 and C9 and forms the membrane attack complex (MAC) as well as the non-lytic fluid-phase SC5b-9 complex (with protein S) . The MAC forms channels in target cell membranes leading to cell lysis by osmotic leakage. The complexes contain neoantigens that are absent from the individual native components from which they are formed and DIA 011-01 is directed against a neoepitope exposed on C9 when incorporated into the TCC.

Synonyms

C5, C5D, C5a, C5b, CPAMD4, ECLZB, complement C5, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4, C5a anaphylatoxin, anaphylatoxin C5a analog, complement component 5, prepro-C5

Gene Name

C5, C6, C7, C8, C9

NCBI Gene ID

727

UniProt

P01031, P13671, P06681, P07357, P07358, P07360, P02749

Host

Mouse

Reactivity

Horse, Human, Non-Human Primate, and Pig

Immunogen

Purified C5b-9

Target

Complement C5b-9

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AE11

Type

Primary Antibodies

Applications

ELISA, IF, IHC, WB

Concentration

1 mg/mL ±15%

Format

Protein A or Protein G purified

Buffer

0.01 M phosphate buffer, pH 7.4, containing 0.15 M NaCl, 0,09% sodium azide

Shipping Conditions

Shipped on ice packs

Storage Conditions

2-8°C without exposure to light

Notes

BSA free

Specificity

DIA 011-01 binds both membrane-bound MAC (active) and fluid-phase SC5b-9 complexes (inactive) . (1) DIA 011-01 binds to a neoepitope exposed on C9

Brand

Antibodies Inc (via BioPorto acquisition)

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Human

Frequently Asked Questions

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