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Anti-Fibronectin Antibody

Our mouse Fibronectin monoclonal antibody from Antibodies Incorporated is produced in-house from clone A32. It detects bovine and human Fibronectin and is Protein A or Protein G purified. Works in ELISA, IHC, IP, WB.

Product Specifications

Background

Fibronectin is an adhesive glycoprotein with a molecular mass of 440 kDa. It is believed to be important for the formation of a provisional matrix that promotes cell adhesion and migration during wound healing. Its age-dependent increase in plasma and tissues may be accompanied in pathological states, especially in tumor growth, by its proteolytic breakdown by a number of neutral proteases. It has also shown that several of its proteolytic breakdown products exhibit unexpected and mostly harmful biological activities. (1)

Synonyms

FN1, fibronectin 1

Gene Name

FN1

NCBI Gene ID

280794

UniProt

P07589

Host

Mouse

Reactivity

Bovine and Human

Immunogen

Bovine corneal endothelial cells

Target

Fibronectin

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

A32

Type

Primary Antibodies

Applications

ELISA, IHC, IP, WB

Concentration

1 mg/mL ±15%

Format

Protein A or Protein G purified

Buffer

0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide

Molecular Weight

440 kDa glycoprotein dimer, with monomers running at 260 on SDS-PAGE

Shipping Conditions

Shipped on ice packs

Storage Conditions

2-8°C without exposure to light

Notes

BSA free

Specificity

CSI 005-32 is highly specific for fibronectin. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, elastin, collagen, laminin) . The antibody inhibits cell adhesion to fibronectin mediated by the heparin-binding domain. Epitope is located in the 40kD Hep II heparin-binding domain.

Brand

Antibodies Inc (via BioPorto acquisition)

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Bovine

Frequently Asked Questions

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