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Anti-Thrombospondin 1 Antibody

Our mouse Thrombospondin 1 monoclonal antibody from Antibodies Incorporated is produced in-house from clone A65M. It detects bovine and human Thrombospondin 1 and is Protein A or Protein G purified. Works in ELISA, IHC, IP.

Product Specifications

Background

Thrombospondin 1 (TSP1) is a multidomain, multifunctional glycoprotein from platelets and certain vascular cells which has been shown to play an important role in cell-cell and cell-matrix interactions. TSP1 is a calcium-sensitive, disulfide-bonded trimer with a subunit molecular mass of 150 kDa. TSP1 exists in different conformational states depending on the calcium ion concentration used in the purification buffers. (1)

Synonyms

THBS1, THBS, THBS-1, TSP, TSP-1, TSP1, thrombospondin-1, glycoprotein G, thrombospondin-1p180, thrombospondin-p50

Gene Name

THBS1

NCBI Gene ID

7057

UniProt

P07996

Host

Mouse

Reactivity

Bovine and Human

Immunogen

Extracellular matrix material from cultured bovine corneal endothelial cells

Target

Thrombospondin 1

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

A65M

Type

Primary Antibodies

Applications

ELISA, IHC, IP

Concentration

1 mg/mL ±15%

Format

Protein A or Protein G purified

Buffer

0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide

Molecular Weight

129 kDA, appears at 150 kDa on WB due to glycosylation

Shipping Conditions

Shipped on ice packs

Storage Conditions

2-8°C without exposure to light

Notes

BSA free

Specificity

CSI 002-65 is highly specific for the low-calcium (0.1 mmol/L) conformation of human TSP1. There is no evidence of cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin) . Epitope is only present in thrombospondin prepared in low-calcium (0.1 mmol/L) buffers.

Brand

Antibodies Inc (via BioPorto acquisition)

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Bovine

Frequently Asked Questions

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