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Anti-Ghrelin Antibody

Our mouse Ghrelin monoclonal antibody from Antibodies Incorporated is produced in-house from clone 45. It detects human and rat Ghrelin and is Protein A or Protein G purified. Works in ELISA, WB.

Product Specifications

Background

Ghrelin is an orexigenic (food intake stimulating) 27 or 28 amino-acid gut-brain peptide hormone produced by endocrine cells in the stomach, pancreatic islets and certain hypothalamic neurons. Two forms are produced by alternative mRNA splicing; Gln13 or 14 is missing from the second form. "Active" ghrelin is acylated (octanoylated) at Ser3; this form stimulates growth hormone secretion via GHS receptors and increases appetite. The des-acyl form does not have these effects but may have other actions. Plasma responses of total ghrelin to food may reflect those of active ghrelin.

Synonyms

GHRL, MTLRP, appetite-regulating hormone, In2c-preproghrelin, ghrelin, growth hormone secretagogue receptor ligand, ghrelin/obestatin preprohormone, ghrelin/obestatin prepropeptide, growth hormone-releasing peptide, motilin-related peptide, prepro-appetite regulatory hormone, preproghrelin

Gene Name

GHRL

NCBI Gene ID

51738

UniProt

Q9UBU3

Host

Mouse

Reactivity

Human and Rat

Immunogen

Synthetic human Ser3-octanoyl ghrelin-28

Target

Ghrelin

Clonality

Monoclonal

Isotype

IgG1 λ

Clone

45

Type

Primary Antibodies

Applications

ELISA, WB

Concentration

1 mg/mL ±15%

Format

Protein A or Protein G purified

Buffer

0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide

Molecular Weight

13 kDa (pro-ghrelin), 6 kDa (mature ghrelin)

Shipping Conditions

Shipped on ice packs

Storage Conditions

2-8°C without exposure to light

Notes

BSA free

Specificity

ABS 050-45 binds human octanoylated ghrelin and human des-acyl ghrelin.

Brand

Antibodies Inc (via BioPorto acquisition)

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Human

Frequently Asked Questions

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