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AM2-10 Nerve Terminal Dye

Our AM2-10 Nerve Terminal Dye is a fixable analog of SynaptoGreenTM C2, a widely used green fluorescent dye for following synaptic activity by staining synaptic vesicles at the synapse or neuromuscular junctions.

Product Specifications

Background

Nerve terminal dyes have highly hydrophilic, cationically charged head groups at one end with lipophilic tails at the other end. They are virtually non-fluorescent in aqueous solution, but become intensely fluorescent in membranes. Following nerve stimulation, the dye molecules are internalized in newly formed endocytic vesicles. During exocytosis, the dyes are released from the vesicles along with neurotransmitters, causing a decrease in fluorescence signal. As a result, the change in fluorescent intensity reflects the amount of endocytosis/exocytosis or synaptic activity. AM2-10 is a fixable analog of SynaptoGreenTM C2.

Type

Dyes & Tracing Reagents

Applications

ICC, IHC, EM

Molecular Weight

503.5 Da

Shipping Conditions

Shipped at ambient temperature with desiccant; protected from light

Storage Conditions

Store at 2°C to 8°C, with desiccant; protect from light.

Specificity

For use in real-time imaging of live/intact cells and cells fixed after staining in formaldehyde.

Recommended Usage

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Physical Properties

Solid

Conjugate

Ex: 480 nm, Em: 598 nm (in membranes)

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