Anti-Notch1 Antibody FL490 Conjugate
Our Anti-Notch1 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N253/32. It detects mouse and rat Notch1, and is purified by Protein A chromatography. It is great for use in ICC.
Product Specifications
Background
Notch Receptor 1 is encoded by the gene NOTCH1. NOTCH1 is a member of the NOTCH family. NOTCH1 is involved in processes related to cell fate specification, differentiation, proliferation, and survival. NOTCH1 is highly expressed in the brain, lungs, and thymus. Diseases associated with NOTCH1 include Aortic Valve Disease 1 and Adams-Oliver Syndrome 5.
Short Description
Our Anti-Notch1 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N253/32. It detects mouse and rat Notch1, and is purified by protein a chromatography. It is great for use in ICC.
Synonyms
Neurogenic locus notch homolog protein 1 (Notch 1) (Motch A) (mT14) (p300) [Cleaved into: Notch 1 extracellular truncation (NEXT) ; Notch 1 intracellular domain (NICD) ]
Gene Name
Notch1 Motch
UniProt
Q01705
Host
Mouse
Reactivity
Mouse and Rat
Immunogen
Fusion protein amino acids 20-216 (extracellular N-terminus, EGF-like domains 1-5) of mouse Notch1 (accession number Q01705) produced recombinantly in E. Coli
Target
Notch1
Clonality
Monoclonal
Isotype
IgG1
Clone
N253/32
Type
Primary Antibody
Applications
ICC
Field of Research
Neurogenesis and Development
Concentration
0.5 mg/mL
Format
Purified by Protein A chromatography
Buffer
PBS with 0.09% azide
Molecular Weight
>270 kDa, ~120 kDa and smaller fragments (due to proteolysis)
Shipping Conditions
Shipped on ice packs
Storage Conditions
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Notes
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and conjugation of purified mAb. Purified mAbs are >90% specific antibody.
Specificity
Does not cross-react with Notch2 or Notch3
Quality Control
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
Recommended Usage
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
Physical Properties
Liquid
Immunogen Species
Mouse
WB Brain Quality Control
1
Conjugate
FL490 Ex: 491 nm, Em: 515 nm
RRID
AB_2940036
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