Anti-Botch Antibody

Our Anti-Botch mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N116/14. It detects human, mouse, and rat Botch, and is purified by Protein A chromatography. It is great for use in ICC, WB.

Product Specifications

Background

Cation transport regulator-like protein 1, Glutathione-Specific Gamma-Glutamylcyclotransferase 1 or Botch1 is encoded by the gene CHAC1. Chac1 is a member of the gamma-glutamylcyclotransferase family. It is an enzyme that acts to catalyze the cleavage of glutathione into 5-oxo-L-proline and a Cys-Gly dipeptide. Chac1 is involved in neuronal differentiation by deglycination of the Notch receptor, which prevents receptor maturation and inhibits Notch signaling. CHAC1 is expressed in tissues including brain (forebrain and anterior spinal cord), and heart. Diseases associated with CHAC1 include Ovarian Embryonal Carcinoma and Ovarian Cancer.

Short Description

Our Anti-Botch mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N116/14. It detects human, mouse, and rat Botch, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, ELISA.

Synonyms

Glutathione-specific gamma-glutamylcyclotransferase 1 (Gamma-GCG 1) (EC 4.3.2.7) (Blocks Notch protein) (Botch) (Cation transport regulator-like protein 1) (Neuroprotective protein 7)

Gene Name

Chac1 Botch Npg7

UniProt

B3STU3

Host

Mouse

Reactivity

Human, Mouse, and Rat

Immunogen

Fusion protein amino acids 1-222 (full-length) of rat Botch1 (accession number B3STU3.1) produced recombinantly in E. Coli

Target

Botch

Clonality

Monoclonal

Isotype

IgG1

Clone

N116/14

Type

Primary Antibody

Applications

WB, IHC, ICC, ELISA

Field of Research

Neurogenesis and Development

Concentration

1 mg/mL

Format

Purified by Protein A chromatography

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.109

Molecular Weight

25 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.