Anti-MESD Antibody

Our Anti-MESD mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N88/12. It detects human, mouse, and rat MESD, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.

Product Specifications

Background

Mesoderm Development LRP Chaperone is encoded by the gene MESD. MESD is a member of the MESD family. MESD is a chaperone specifically assisting the folding of beta-propeller/EGF modules within the family of low-density lipoprotein receptors (LDLRs) . MESD acts as a modulator of the Wnt pathway through chaperoning the coreceptors of the canonical Wnt pathway, LRP5 and LRP6, to the plasma membrane. MESD is essential for the specification of embryonic polarity and mesoderm induction. MESD plays an essential role neuromuscular junction (NMJ) formation by promoting cell-surface expression of LRP4. MESD is expressed in many tissues, but not in skeletal muscles. Diseases associated with MESD include Osteogenesis Imperfecta, Type Xx and Epilepsy, Nocturnal Frontal Lobe, 2.

Short Description

Our Anti-MESD mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N88/12. It detects human, mouse, and rat MESD, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, IP, ELISA.

Synonyms

LRP chaperone MESD (LDLR chaperone MESD) (Mesoderm development candidate 2) (Mesoderm development protein)

Gene Name

Mesd Mesdc2

UniProt

Q9ERE7

Host

Mouse

Reactivity

Human, Mouse, and Rat

Immunogen

Fusion protein amino acids 30-95 and 118-180 (lacking VSD domain) of mouse MESD (accession number Q9ERE7) produced recombinantly in E. Coli

Target

MESD

Clonality

Monoclonal

Isotype

IgG2b

Clone

N88/12

Type

Primary Antibody

Applications

WB, IHC, ICC, IP, ELISA

Field of Research

Cell Signaling/Wnt Signaling

Concentration

1 mg/mL

Format

Purified by Protein A chromatography

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.56

Molecular Weight

26 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.