JC-1

JC-1 (CBIC2) is an ideal fluorescent probe widely used to detect mitochondrial membrane potential. JC-1 accumulates in mitochondria in a potential dependent manner and can be used to detect the membrane potential of cells, tissues or purified mitochondria. In normal mitochondria, JC-1 aggregates in the mitochondrial matrix to form a polymer, which emits strong red fluorescence (Ex=585 nm, Em=590 nm) ; When the mitochondrial membrane potential is low, JC-1 cannot aggregate in the matrix of mitochondria and produce green fluorescence (ex=510 nm, em= 527 nm) [1].

Product Specifications

CAS Number

[3520-43-2]

Product Name Alternative

CBIC2

UNSPSC

12171500

Hazard Statement

H302, H315, H319, H335, H410

Target

Fluorescent Dye

Type

Dye Reagents

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/JC-1.html

Purity

99.0

Solubility

DMSO : 5 mg/mL (ultrasonic; warming) |H2O : < 0.1 mg/mL

Smiles

ClC1=C(Cl)C=C([N+](CC)=C(/C=C/C=C2N(C(C=C(Cl)C(Cl)=C3)=C3N\2CC)CC)N4CC)C4=C1.[I-]

Molecular Formula

C25H27Cl4IN4

Molecular Weight

652.23

Precautions

H302, H315, H319, H335, H410

References & Citations

[1]A Perelman, et al. JC-1: alternative excitation wavelengths facilitate mitochondrial membrane potential cytometry. Cell Death Dis. 2012 Nov 22;3:e430.|[2]Vera C. Keil, et al. Ratiometric high-resolution imaging of JC-1 fluorescence reveals the subcellular heterogeneity of astrocytic mitochondria. Pflügers Archiv - European Journal of Physiology. 2011,462 (5) : 693-708.|[3]Jung-Ho LEE, et al. Real-time analysis of amyloid fibril formation of α-synuclein using a fibrillation-state-specific fluorescent probe of JC-1. Biochem. J. 2009, 418:311-323.|[4]Salvioli S, et al. JC-1, but not DiOC6 (3) or rhodamine 123, is a reliable fluorescent probe to assess delta psi changes in intact cells: implications for studies on mitochondrial functionality during apoptosis. FEBS Lett. 1997 Jul 7;411 (1) :77-82.