Rat MCP-1 PicoKine® Quick ELISA Kit

Monocyte chemoattractant protein-1 (MCP-1), a member of the chemokine (chemotactic cytokine) family, is a potent monocyte agonist that is upregulated by oxidized lipids.1 MCP-1 is also known as CCL2, SCYA2, MCAF. MCAF is a member of family of factors involved in immune and inflammatory responses. The amino acid sequence deduced from the nucleotide sequence reveals the primary structure of the MCAF precursor to be composed of a putative signal peptide sequence of 23 amino acid residues and a mature MCAF sequence of 76 amino acid residues.2 MCP-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder.3

C C motif chemokine 2; CCL2; chemokine (C C motif) ligand 2; GDCF 2; HC11; HSMCR30; MCAF; MCP 1; MCP1; Monocyte chemotactic protein 1; Monocyte secretory protein JE; SCYA2; Small inducible cytokine A2; SMC CF

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 26 68 361 28 121 468 Standard deviation 0.79 3.76 21.84 1.27 4.85 36.13 CV (%) 3.0% 5.5% 6.1% 4.5% 4.0% 7.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 26 68 361 28 121 468 Standard deviation 0.79 3.76 21.84 1.27 4.85 36.13 CV (%) 3.0% 5.5% 6.1% 4.5% 4.0% 7.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 26 68 361 28 121 468 Mean (pg/ml) 26 68 361 28 121 468 Standard deviation 0.79 3.76 21.84 1.27 4.85 36.13 Standard deviation 0.79 3.76 21.84 1.27 4.85 36.13 CV (%) 3.0% 5.5% 6.1% 4.5% 4.0% 7.7% CV (%) 3.0% 5.5% 6.1% 4.5% 4.0% 7.7%

Description|Quantity 96-well plate|1 lyophilized Standard| Biotinylated detection antibody (50x) |100 ul Avidin-Biotin-Peroxidase Complex (30x) |400 μl Sample diluent|30 ml Antibody diluent|12 ml Avidin-Biotin-Peroxidase Diluent|12 ml Wash Buffer (25x) |20 ml Color Developing Reagent (TMB) |10 ml Stop Solution|10 ml Plate Sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.009 0.054 0.094 0.176 0.302 0.581 1.106 1.989 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.009 0.054 0.094 0.176 0.302 0.581 1.106 1.989 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.009 0.054 0.094 0.176 0.302 0.581 1.106 1.989 O.D. 0.009 0.054 0.094 0.176 0.302 0.581 1.106 1.989

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.