Urease Inhibitor Screening Kit (Colorimetric)

Urease (Urea Aminohydrolase, E.C.3.5.1.5) is a nickel-dependent metalloenzyme that catalyzes the hydrolysis of urea to ammonia and carbon dioxide. Urease is produced by plants, bacteria, fungi and some invertebrates. Bacterial urease is of special importance since it is a major virulence factor caused by bacteria during infections. It is essential in the colonization of host organism and in the maintenance of bacterial cells in tissues. Ureases hydrolyze urea secreted in the blood and saliva to release ammonia, which is the major factor contributing to infections such as urinary tract and gastroduodenal infections, urinary catheter encrustation, stomach cancer, kidney stone formation, pneumonia etc. Thus, controlling the ureolytic activity of microorganism is a promising target to treat diseases caused by bacterial ureases. BioVision’s Urease Inhibitor Screening Kit provides a quick, easy and sensitive assay to screen and characterize potential urease inhibitors. In this assay, urease of Canavalia ensiformis is used to screen urease inhibitors. The production of ammonia is measured at 670 nm using a modified Berthelot method and is applied to determine the inhibition efficiency of the screened compounds. A control Urease Inhibitor is also included in the kit.