PGE2 Competitive ELISA Kit PicoKine®

Prostaglandin E2 (PGE2), also known as dinoprostone, is a naturally occurring prostaglandin with oxytocic properties that is used as a medication.Dinoprostone is used in labor induction, bleeding after delivery, termination of pregnancy, and in newborn babies to keep the ductus arteriosus open.In babies it is used in those with congenital heart defects until surgery can be carried out. It is also used to manage gestational trophoblastic disease.It may be used within the vagina or by injection into a vein.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 6 153 3359 9 123 19358 Standard deviation 0.37 11.26 192.13 0.50 9.24 1288.03 CV (%) 6.1% 7.4% 5.7% 5.5% 7.5% 6.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 6 153 3359 9 123 19358 Standard deviation 0.37 11.26 192.13 0.50 9.24 1288.03 CV (%) 6.1% 7.4% 5.7% 5.5% 7.5% 6.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 6 153 3359 9 123 19358 Mean (pg/ml) 6 153 3359 9 123 19358 Standard deviation 0.37 11.26 192.13 0.50 9.24 1288.03 Standard deviation 0.37 11.26 192.13 0.50 9.24 1288.03 CV (%) 6.1% 7.4% 5.7% 5.5% 7.5% 6.6% CV (%) 6.1% 7.4% 5.7% 5.5% 7.5% 6.6%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials HRP conjugated antigen (10ug/ml*60 ul) |1 vials Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 2 20 200 2000 20000 O.D. 2.301 2 1.88 1.193 0.812 0.387 Concentration (pg/ml) 0 2 20 200 2000 20000 O.D. 2.301 2 1.88 1.193 0.812 0.387 Concentration (pg/ml) 0 2 20 200 2000 20000 Concentration (pg/ml) 0 2 20 200 2000 20000 O.D. 2.301 2 1.88 1.193 0.812 0.387 O.D. 2.301 2 1.88 1.193 0.812 0.387

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.