What sizes are available for Rabbit anti Human Sphingosine 1 Phosphate 3 Receptor (EDG-3) NT?

Rabbit anti Human Sphingosine 1 Phosphate 3 Receptor (EDG-3) NT is available in the following size(s): 100 µg.

Is this product animal-origin-free?

Animal-origin-free status, where applicable, is noted on the datasheet under formulation details.

Does this product need any special customs documentation to be imported to my country?

If you’re outside the EU, UK, or USA, you’re responsible for customs clearance and any related taxes or fees. We provide the standard import documents, such as the Commercial Invoice, Packing List, and Airway Bill, in advance, so let us know early if your customs office needs anything additional for this specific product.

What buffer is this product supplied in?

The formulation or storage buffer is listed on the product datasheet. If you require a carrier-free or custom formulation, contact us before ordering, as this may involve a made-to-order production timeline.

Rabbit anti Human Sphingosine 1 Phosphate 3 Receptor (EDG-3) NT

Q: What is the catalog number for Rabbit anti Human Sphingosine 1 Phosphate 3 Receptor (EDG-3) NT?

The catalog number for Rabbit anti Human Sphingosine 1 Phosphate 3 Receptor (EDG-3) NT is X1590P.

Product Specifications

Background

Endothelial cell differentation gene-3 (EDG3) belongs to a family of G-protein couple receptors whose ligands are lysophospholipids. The ligand for EDG3 is sphingosine-1-phosphate. There are 8 known members of the EDG receptor family. They have been implicated in mediating growth related effects such as induction of cellular proliferation, alterations in differentiation and survival suppression of apoptosis. They also evoke cellular effector functions that are dependent on cytoskeletal responses such as contraction, secretion, adhesion and chemotaxis. EDG receptors are developmentally regulated and differ in tissue distribution. The couple to multiple types of G proteins to signal through ras and MAP kinase, rho, phospholipase C and several protein tyrosine kinases. EDG3 is expressed in cardiovascular, leukocyte-containing and other tissues.

CAS Number

9007-83-4

Synonyms

Endothelial Cell differentiation gene 3 N-terminal, Sphingosine-1-Phosphate Receptor-3 (S1P3)

UniProt

Q99500

Host

Rabbit

Species Reactivity

Human,Mouse

Conjugation

Unconjugated

Type

Polyclonal Antibody

Source

Rabbits were immunized with a synthetic peptide derived from the N terminal of the EDG3 (S1P3) protein.

Applications

WB, IP

Field of Research

Signal Transduction

Purification Method

Ammonium Sulfate Precipitation

Assay Principle

Antibody can be used for Western blotting (5-10 µg/mL) and Immunoprecipitation (see reference 4). Optimal concentration should be evaluated by serial dilutions. Due to low expression of EDG receptors, we recommend use of Pierce Femto Signal substrate for western blot development.

Stability

See expiration date on vial

Concentration

See vial for Concentration

Form

Provided as solution in phosphate buffered saline with 0,08% sodium azide

Precautions

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

References & Citations

1. Goetzl EJ and An, S. Diversity of cellular receptors and functions for the lysophospholipid growth factos lysophosphatidic acid and sphingosine 1-phosphate. FASEB J. 1998: 12, 1589-98._x000D_ 2. An, S., Goetzl, E.J., Lee, H. Signaling mechanisms and molecular characteristics of G protein-coupled receptors for lysophosphatidic acid and sphingosine 1-phosphate. J. Cell Biochem. Suppl. 1998: 30-31, 147-57._x000D_ _x000D_ 3. Singleton PA, Moreno-Vinasco L, Sammani S, Wanderling SL, Moss J, Garcia JG. Attenuation of vascular permeability by methylnaltrexone: role of mOP-R and S1P3 transactivation. Am J Respir Cell Mol Biol. 2007;37(2):222-31._x000D_ _x000D_ 4. Sun X, Singleton PA, Letsiou E, Zhao J, Belvitch P, Sammani S, Chiang ET, Moreno-Vinasco L, Wade MS, Zhou T, Liu B, Parastatidis I, Thomson L, Ischiropoulos H, Natarajan V, Jacobson JR, Machado RF, Dudek SM, Garcia JG. Sphingosine-1-phosphate receptor-3 is a novel biomarker in acute lung injury. Am J Respir Cell Mol Biol. 2012 Nov;47(5):628-36. doi: 10.1165