Human NT-proBNP/NPPB ELISA Kit PicoKine®

NPPB is a member of the natriuretic peptide family and encodes a secreted protein which functions as a cardiac hormone. The protein undergoes two cleavage events, one within the cell and a second after secretion into the blood. The protein's biological actions include natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion, and a key role in cardiovascular homeostasis. A high concentration of this protein in the bloodstream is indicative of heart failure.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 165 827 2641 181 779 2720 Standard deviation 3.61 41.18 200.98 8.87 59.83 110.70 CV (%) 2.2% 5.0% 7.6% 4.9% 7.7% 4.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 165 827 2641 181 779 2720 Standard deviation 3.61 41.18 200.98 8.87 59.83 110.70 CV (%) 2.2% 5.0% 7.6% 4.9% 7.7% 4.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 165 827 2641 181 779 2720 Mean (pg/ml) 165 827 2641 181 779 2720 Standard deviation 3.61 41.18 200.98 8.87 59.83 110.70 Standard deviation 3.61 41.18 200.98 8.87 59.83 110.70 CV (%) 2.2% 5.0% 7.6% 4.9% 7.7% 4.1% CV (%) 2.2% 5.0% 7.6% 4.9% 7.7% 4.1%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.034 0.074 0.1211 0.207 0.341 0.644 1.198 2.485 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.034 0.074 0.1211 0.207 0.341 0.644 1.198 2.485 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.034 0.074 0.1211 0.207 0.341 0.644 1.198 2.485 O.D. 0.034 0.074 0.1211 0.207 0.341 0.644 1.198 2.485

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.