Mouse CXCL9/Mig PicoKine® Quick ELISA Kit

Chemokine (C-X-C motif) ligand 9 (CXCL9) is a small cytokine belonging to the CXC chemokine family that is also known as Monokine induced by gamma interferon (MIG) . CXCL9 is a T-cell chemoattractant, which is induced by IFN-γ. It is closely related to two other CXC chemokines called CXCL10 and CXCL11, whose genes are located near the gene for CXCL9 on human chromosome 4. CXCL9, CXCL10 and CXCL11 all elicit their chemotactic functions by interacting with the chemokine receptor CXCR3. The standard product used in this kit is recombinant human CXCL9, consisting of 103 amino acids with the molecular mass of 11.7KDa.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 76 372 1204 108 360 1271 Standard deviation 4.95 9.19 39.13 4.61 19.58 89.99 CV (%) 6.5% 2.5% 3.3% 4.3% 5.4% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 76 372 1204 108 360 1271 Standard deviation 4.95 9.19 39.13 4.61 19.58 89.99 CV (%) 6.5% 2.5% 3.3% 4.3% 5.4% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 76 372 1204 108 360 1271 Mean (pg/ml) 76 372 1204 108 360 1271 Standard deviation 4.95 9.19 39.13 4.61 19.58 89.99 Standard deviation 4.95 9.19 39.13 4.61 19.58 89.99 CV (%) 6.5% 2.5% 3.3% 4.3% 5.4% 7.1% CV (%) 6.5% 2.5% 3.3% 4.3% 5.4% 7.1%

Description|Quantity|Volume Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 HRP-Linked Antibody|1|6ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.018 0.037 0.066 0.116 0.239 0.47 0.996 2.188 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.018 0.037 0.066 0.116 0.239 0.47 0.996 2.188 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.018 0.037 0.066 0.116 0.239 0.47 0.996 2.188 O.D. 0.018 0.037 0.066 0.116 0.239 0.47 0.996 2.188

Aliquot 0.1 ml per well of the 4,000 pg/ml, 2,000 pg/ml, 1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml mouse CXCL9 standard solutions into the precoated 96-well plate. Add 0.1 ml of the sample diluent buffer into the control well (Zero well) . Add 0.1 ml of each properly diluted sample of mouse cell culture supernatants, serum or plasma (EDTA) to each empty well. See “Sample Dilution Guideline” above for details. It is recommended that each mouse CXCL9 standard solution and each sample be measured in duplicate.

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.