Human Fetuin A/AHSG PicoKine® Quick ELISA Kit

Alpha2-HS glycoprotein (AHSG), also known as fetuin-A, is a plasma protein displaying high-affinity interaction with calcium phosphate, by which ectopic vascular calcification is prevented. The AHSG polymorphism is attributable to the hereditary variation of AHSG and phosphate serum levels, which may affect skeletal development and chronic disorders such as vascular calcification.1Human plasma protein alpha 2-HS-glycoprotein (AHSG) is composed of two polypeptide chains, A and B, encoded by a single mRNA. Southern blot analysis of mouse x human somatic cell hybrids has mapped the AHSG gene to human chromosome 3 in the region 3q21----qter. Using a recombinant plasmid containing a 1,538 bp insert spanning the entire AHSG coding region, AHSG was localized to chromosomal bands 3q27----q29 by in situ hybridization.2

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (ng/ml) 1 6 20 1 5 13 Standard deviation 0.05 0.21 1.42 0.05 0.35 0.70 CV (%) 4.5% 3.6% 7.1% 4.8% 7.0% 5.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (ng/ml) 1 6 20 1 5 13 Standard deviation 0.05 0.21 1.42 0.05 0.35 0.70 CV (%) 4.5% 3.6% 7.1% 4.8% 7.0% 5.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (ng/ml) 1 6 20 1 5 13 Mean (ng/ml) 1 6 20 1 5 13 Standard deviation 0.05 0.21 1.42 0.05 0.35 0.70 Standard deviation 0.05 0.21 1.42 0.05 0.35 0.70 CV (%) 4.5% 3.6% 7.1% 4.8% 7.0% 5.4% CV (%) 4.5% 3.6% 7.1% 4.8% 7.0% 5.4%

Description|Quantity|Volume Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 HRP-Linked Antibody|1|6ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.010 0.033 0.059 0.123 0.245 0.518 1.036 1.897 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.010 0.033 0.059 0.123 0.245 0.518 1.036 1.897 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.010 0.033 0.059 0.123 0.245 0.518 1.036 1.897 O.D. 0.010 0.033 0.059 0.123 0.245 0.518 1.036 1.897

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.